重庆医学2012，Vol.41Issue(20)：2009-2011,2014,4.DOI:10.3969/j.issn.1671-8348.2012.20.001
远志等中药对脑淀粉样血管病相关蛋白胱抑素聚集的影响
Effect of Polygala on the aggregation of cerebral amyloid disease-related protein cystatin
摘要
Abstract
Objective The primary aim of this study is to investigate the effect of traditional Chinese medicine on the aggregation of Human cystatin C,a kind of cerebral amyloid disease-related protein. Methods With the addition of suboptimal concentrations of Chinese medicine to the induction medium of cystatin yeast expression system, the effects of traditional Chinese medicine on cystatin is determined both in vilro and in vivo using SDS-PAGE and western blotting. Results Extracellular experiments demonstrated that Polygala significantly represses the aggregation of cystatin. The concentration of Polygala has a positive relationship to its inhibitory effect on cystatin aggregation at the concentration below 500 μg/mL. Intracellular experiments showed that Polygala has no effect on cystatin aggregation in vivo. Conclusion Polygala has an obvious inhibitory effect on the aggregation of secreted cystatin.关键词
脑淀粉样血管病/远志/胱抑素/蛋白聚集Key words
cerebral amyloid angiopathy/polygala tenuifolia/cystatin/protein aggregation引用本文复制引用
何剑为,马婧思,于东润,张朔,邹志远,宋有涛..远志等中药对脑淀粉样血管病相关蛋白胱抑素聚集的影响[J].重庆医学,2012,41(20):2009-2011,2014,4.基金项目
国家自然科学基金资助项目(30970152) （30970152）
辽宁省教育厅优秀人才项目(2009R26) （2009R26）
教育部留学回国人员科研启动基金资助项目(教外司留[2010] （）
