重庆医学2012，Vol.41Issue(20)：2062-2063,2067,3.DOI:10.3969/j.issn.1671-8348.2012.20.022
急性心肌梗死后快速延迟整流K+通道基因表达的变化
Alteration in gene expression of the rapid-delayed rectifier potassium channel after acute myocardial infarction
李秀娟 1黄从新 2丁家望 1杨俊 1吴辉 1李莉 1李松 1李稳慧 1姜玉蓉1
作者信息
- 1. 三峡大学第一临床学院/宜昌市中心人民医院心内科,湖北宜昌,443003
- 2. 武汉大学人民医院心内科,武汉,430000
- 折叠
摘要
Abstract
Objective To investigate the changes and significance of the expression of the rapid-delayed rectifier potassium channel KCNH2 and KCNE2 mRNA in infarcted marginal zone after myocardial infarction (MI) in pigs. Methods The pig model of AMI was established by ligating left anterior descending coronary artery for 2 h,and the survival porcine were enrolled into post-MI group. 24 h after surgery, using semi-quantitative reverse transcriptase polymerase chain reaction(RT-PCR) , KCNH2 and KC-NE2mRNA were measured respectively in endocardium(Endo) ,midcardium (Mid) and epicardium(Epi) of border zone. Besides,the sham-operation group(sham-group) was established. Results The mRNA of KCNH2 and KCNE2 were similar in endocardium (Endo) ,midcardium (Mid) and epicardium(Epi) in sham group. Compared with the sham-group, KCNH2 mRNA in all the three layers of border zone in AMI group remarkably reduced respectively (P<0. 05) ,and there were significant differences among three layers(P<0. 05) , while the mRNA of KCNE2 was similar among three layers(P>0. 05). Conclusion The heterogeneous downreg-ulation of KCNH2mRNA expression may be one of the molecular mechanisms that are able to cause ventricular arrhythmias after a-cute myocardial infarction.关键词
钾通道/心肌梗死/基因表达/猪Key words
potassium channels/myocardial infarction/gene expression/porcine引用本文复制引用
李秀娟,黄从新,丁家望,杨俊,吴辉,李莉,李松,李稳慧,姜玉蓉..急性心肌梗死后快速延迟整流K+通道基因表达的变化[J].重庆医学,2012,41(20):2062-2063,2067,3.
