重庆医学2012，Vol.41Issue(21)：2131-2133,3.DOI:10.3969/j.issn.1671-8348.2012.21.003
小檗碱对Ang Ⅳ诱导血管平滑肌细胞增殖的影响
Inhibitory effects of berberine on proliferation of vascular smooth muscle cells induced by angiotensin Ⅳ
摘要
Abstract
Objective To investigate the effects of berberine on the proliferation of vascular smooth muscle cells (VSMCs) induced by angiotensin IV (Ang IV),and explore the possible mechanisms related to nitric oxide (NO). Methods Primary VSMCs were cultured by tissue explant method. Cell proliferation were measured by MTT and total protein contents assays to observe the effects of berberine (10,30,100 μmol/L) on VSMCs proliferation induced by Ang IV (0. 1 nmol/L). The expression of endothelial NO synthase (NOS) mRNA was determined by real time RT-PCR method. The NOS activity and NO concentration in culture media were assayed by spectrophotometry and nitrate reduction methods, respectively. Results Berberine remarkably inhibited VSMCs proliferation induced by Ang IV in a dose-dependent manner(P<0. 05) ,and increased the activity of NOS and the contents of NO in cultured cells(P<0. 05). Furthermore,berberine up-regulated the expression level of endothelial NOS mRNA(P<0. 05). L-arginine had a similar effect to berberine(P<0. 05). NG-nitro-L-arginine methyl ester,NOS inhibitor,could abolish above effects of both berberine and L-arginine(P<0. 05). Conclusion Berberine obviously attenuated the cultured VSMCs proliferation induced by AngIV. The mechanisms are,at least partly,due to up-regulating the expression of eNOS,then increasing NOS activity,and promoting the synthesis and release of NO.关键词
小檗碱/血管平滑肌细胞/增殖/血管紧张素Ⅳ/一氧化氮Key words
berberine/vascular smooth muscle cells/proliferation/angiotensin IV /nitric oxide引用本文复制引用
王全华,陈加飞,王平,吴芹,蒋青松..小檗碱对Ang Ⅳ诱导血管平滑肌细胞增殖的影响[J].重庆医学,2012,41(21):2131-2133,3.基金项目
国家自然科学基金资助项目(81100905) （81100905）
重庆市自然科学基金资助项目(2010BB5108). （2010BB5108）
