高温大曲中筛选产纤维素酶的耐高温芽孢杆菌OA北大核心CSCDCSTPCD
Screening of a Thermostable Cellulase-secreting Bacillus from High Temperature Daqu
从高温大曲中筛选出产纤维素酶的芽孢杆菌1株,进行形态特征、生化鉴定和16S rDNA序列分子鉴定,该菌株鉴定为Bacillus cereus Frankland.对该菌株进行产酶摇瓶液体发酵,测定其内切纤维素酶活力和外切纤维素酶活力分别为260.32,87.22 u·mL-1,对该菌株进行产酶固态发酵,测定其内切纤维素酶活力和外切纤维素酶活力分别为556.64.121.47 u· mL-1.酶的稳定性实验显示该菌株产纤维素酶在60℃以下和在pH4…查看全部>>
The Bacillus that can produce cellulase is isolated and purified from the high temperature Daqu,based on the analysis of 16SrDNA and some biochemical and physiological characteristics, this strain was identified as Bacillus cereus Frankland. The shake flask liquid fermentation was taken to determine the endo-cellulase activity (260. 32 u·mL-1), and exo-cellulase activity (87.22 u·mL-1), the solid state fermentation was taken to determine the endo-cellulase a…查看全部>>
龚丽琼;邓朝霞;黄祖新;颜林春;马校卫;汤二将;陈由强;陈如凯
福建师范大学生命科学学院,福建福州350108农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州350108福建省发育与神经生物学重点实验室,福建福州350108福建师范大学生命科学学院,福建福州350108农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州350108福建省发育与神经生物学重点实验室,福建福州350108福建师范大学生命科学学院,福建福州350108农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州350108
生物科学
大曲纤维素酶芽胞杆菌液体发酵固态发酵响应面优化
Daqu cellulase Bacillus liquid fermentation solid state fermentation response surface methodology
《福建师范大学学报（自然科学版）》 2012 (3)
106-112,7
国家现代农业产业技术体系建设专项资金资助项目(CARS-20-4-4)福建省教育厅资助项目(JK2010013 03BC727)