分析测试学报2012，Vol.31Issue(5)：564-569,6.DOI:10.3969/j.issn.1004-4957.2012.05.010
基于L-半胱氨酸修饰的锰掺杂ZnS量子点应用于叶酸测定
Determination of Folic Acid Using Mn2+ Doped ZnS Quantum Dots Capped with L-Cysteine
摘要
Abstract
Water-soluble L-cysteine-modified-ZnS : Mn2+ quantum dots(QDs) were prepared in aqueous solution under the room temperature, and characterized by infrared spectrometry, ultravioletvisible spectrometry, fluorescence spectrometry and X-ray diffraction spectrometry. Based on the flu-orescence quenching of ZnS : Mn2+QDs caused by folic acid (FA) , the prepared QDs were evaluated as a fluorescence probe for FA detection. Factors affecting the fluorescence detection on FA were studied. Under the optimal conditions, the quenched fluorescence intensity was linear with FA con-centration in the range of 1. 0×10-6 - 7.0×10-5 mol L-1 with correlation coefficient of 0.994. The limit of detection for FA was 9. 6× 10 -7 mol L-1. The proposed method was applied in the determination of folic acid in folic acid tablet and urine samples with satisfactory results. The mecha-nism for detection of FA was also investigated.关键词
叶酸/ZnS/Mn2+/荧光测定/L-半胱氨酸Key words
folic acid/ Mn-doped ZnS quantum dots/ fluorescence determination/ L-cysteine分类
化学化工引用本文复制引用
占浔寿,商燕,吴芳英..基于L-半胱氨酸修饰的锰掺杂ZnS量子点应用于叶酸测定[J].分析测试学报,2012,31(5):564-569,6.基金项目
国家自然科学基金资助项目(20965006) （20965006）
