分析测试学报2012，Vol.31Issue(6)：675-679,5.DOI:10.3969/j.issn.1004-4957.2012.06.008
中草药对DNA氧化损伤水平的微分脉冲伏安法测定
Determination of the Levels of DNA Damage Induced by Chinese Herbal Medicine Using Differential Pulse Voltammetry
摘要
Abstract
Effects of DNA damage induced by Chinese herbal medicine were studied by differential pulse voltammetric ( DPV) method. The voltammeitric behaviour of 8-hydorxy-2'-deoxyano-sine ( 8-OHdG) in the phosphate buffer (pH 5. 0) was investigated at a glassy carbon electrode by cyclic voltammetry (CV) and DPV. A well-defined oxidation peak of 8-OHdG at +0. 5 V was found, and its current intensity was proportional to concentration of 8-OHdG in the range of 1. 0×10 -6 - 7. 1 ×10-4 mol/L. The regression equation was Ip((μA) =0.004 3e(mol/L) +4 ×10-8 ( r =0. 999 8) and the detection limit (S/N = 3 ) was 3. 5× 10-7 mol/L. The method was applied in analysis of the levels of 8-OHdG in the calf thymus DNA(ctDNA) exposed to concentration of 40 g/L of glycynhiza, cherokee rose, eucommia ulmoides, pinellia, nux vomica extract for 2 h, respectively, and the blood of Kunming mice exposed to low and high concentration of nux vomica extract by mouth injection for 30 consecutive days. The results showed that glycyrrhiza, cherokee rose, eucommia ulmoides, pinellia ex-tract could not cause ctDNA oxidative damage, and nux vomica extract can cause DNA oxidative dam-age to the formation of 8-OHdG in the average level of (3. 2 0. 2)μmol/L. The average levels of 8-OHdG were (2. 0 0. 1) (μmol/L and (5. 3 0. 3) μmol/L in the blood after a long-term administration of low concentration and high concentration of nux vomica extracts of Kunming mice, respectively. The study indicated that nux vomica contained the potential genotoxicity.关键词
微分脉冲伏安法(DPV)/中草药/DNA氧化损伤/8-羟基脱氧鸟苷(8-OHdG)/遗传毒性Key words
differential pulse voltammetry ( DPV ) / Chinese herbal medicine/ DNA damage/ 8-hydorxy-2'-deoxyano-sine( 8-OHdG) / genotoxicity分类
化学化工引用本文复制引用
利健文,陈任宏,崔丽京,汪小根..中草药对DNA氧化损伤水平的微分脉冲伏安法测定[J].分析测试学报,2012,31(6):675-679,5.基金项目
广东省科技计划项目(2011B020314011) （2011B020314011）
