分析化学2012，Vol.40Issue(5)：699-704,6.DOI:10.3724/SP.J.1096.2012.11085
以局部结构类似物为类模板的分子印迹整体柱在线检测三甲氧苄啶
On-line Determination of Trimethoprim in Human Serum by Molecularly Imprinted Monolithic Column with Melamine Employed as Analogue Template
摘要
Abstract
A molecularly imprinted monolithic column being capable of selectively adsorbing trimethoprim (TMP) was synthesized by in situ polymerization method. During the polymerization process, melamine was employed as analogue template, and mcthacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross linker, polyethylene glycol 400 (PEG400) as porogenic agent, respectively. The change of recognition ability for MIP column towards TMP was validated under different kinds of mobile phase compositions in a high performance liquid chromatographic (HPLC) system. Experimental results showed that MIP column could completely retain TMP under mobile phases of methanol, acetonitrile or water. TMP was selectively adsorbed in MIP column under metha-nol-water (80:20, V/V) , then the hydrophobic impurities were further erased by changing the mobile phase to methanol, and finaly, the TMP peak was obtained in the elution step of with ammonia water-methanol (2. 5: 97. 5, V/V). Online determination of TMP in human serum was performed in HPLC system with the imprinted column employed as chromatography column. The linear regression equation obtained was determined as A = 42. 8c -3. 03(r = 0. 9994) with the linear range of 8. 32-93. 8 mg/L and a limit of detection of 0. 145 mg/L.关键词
三甲氧苄啶/分子印迹整体柱/类模板/三聚氰胺/高效液相色谱/在线检测Key words
Trimethoprim/ Molecularly imprinted monolithic column/ Analogue template/ Melamine, High performance liquid chromatography/ On-line determination引用本文复制引用
张红武,郭建文,李康,王伟声,吴玉萍,卢绮雯,翟海云..以局部结构类似物为类模板的分子印迹整体柱在线检测三甲氧苄啶[J].分析化学,2012,40(5):699-704,6.基金项目
本文系国家自然青年科学基金(No.21005021)资助项目 （No.21005021）
