分析化学2012，Vol.40Issue(5)：713-717,5.DOI:10.3724/SP.J.1096.2012.11025
代谢组学方法研究姜黄根茎及块根次生代谢产物表达差异
Metabonomic Study on Secondary Metabolites of Rhizome and Tuberous Root of Curcuma longa L
摘要
Abstract
A metabonomic method of GC-MS was developed to investigate the difference of secondary metabolites in the rhizome and tuberous root of Curcuma longa L. The extraction conditions, temperature program and methodology were optimized. Using the petroleum ether as the extraction solvent and Soxhlet extraction, the method displayed good precision(RSD: 1. 2% -5. 7%), repea-tabilityCRSD: 2. 2%-6. 3%) and linearity (R2 . 0. 699-0972). The analysis data were performed by SIMCA-P software. Scores plot of PCA could successfully divide the rhizome and tuberous root samples into two groups respectively, which illustrate the difference of secondary metabolites between the rhizome and tuberous root. With the loading plot and t-test, the significant difference in two groups of fourteen endogenous metabolites were found, which may be the effective substance for explaining the different usage of the rhizome and tuberous root of Curcuma longa L. in Chinese medicine.关键词
次生代谢物/姜黄/气相色谱-质谱/主成分分析Key words
Secondary metabolites/ Curcuma. Longa L/ Gas chromatography-mass spectrometry/ Principal component analysis引用本文复制引用
吴宏伟,李洪梅,唐力英,许海玉,李韶菁,唐仕欢,杨洪军,邵爱娟,黄璐琦..代谢组学方法研究姜黄根茎及块根次生代谢产物表达差异[J].分析化学,2012,40(5):713-717,5.基金项目
本文系科技部国际合作项目(No.2009DFA31660)和973项目(No.2006CB504701)资助 （No.2009DFA31660）
