法医学杂志2012，Vol.28Issue(2)：85-88,91,5.DOI:10.3969/j.issn.1004-5619.2012.02.002
肠道病毒71型在脑干脑炎婴幼儿脑干中的分布及感染机制
Distribution of Human Enterovirus 71 in Brainstem of Infants with Brain Stem Encephalitis and Infection Mechanism
摘要
Abstract
Objective To explore the mechanism that how human enterovirus 71(EV71) invades the brain-stem and how intercellular adhesion molecules-1 (ICAM-1) participates by analyzing the expression and distribution of human EV71, and ICAM-1 in brainstem of infants with brain stem encephalitis. Methods Twenty-two brainstem of infants with brain stem encephalitis were collected as the experimental group and 10 brainstems of fatal congenital heart disease were selected as the control group. The sections with perivascular cuffings were selected to observe EV71-VP1 expression by immunohistochemistry method and ICAM-1 expression was detected for the sections with EV71-VP1 positive expression. The staining image analysis and statistics analysis were performed. The experiment and control groups were compared. Results (1) EV71-VP1 positive cells in the experimental group were mainly astrocytes in brainstem with nigger-brown particles, and the control group was negative. (2) ICAM-1 positive cells showed nigger-brown. The expression in inflammatory cells (around blood vessels of brain stem and in glial nodules) and gliocytes increased. The results showed statistical difference comparing with control group (P<0.05). Conclusion The brainstem encephalitis can be used to diagnose fatal EV71 infection in infants. EV71 can invade the brainstem via hematogenous route. ICAM-1 may play an important role in the pathogenic process.关键词
法医病理学/手足口病/肠道病毒属/细胞间黏附分子1Key words
forensic pathology/ hand, foot and mouth disease/ enterovirus/ intercellular adhesion molecules-1分类
社会科学引用本文复制引用
郝博,权力,罗斌,高迪,汤大为,王小广,刘水平,孔小平,刘超,黄京璐,毕启明..肠道病毒71型在脑干脑炎婴幼儿脑干中的分布及感染机制[J].法医学杂志,2012,28(2):85-88,91,5.基金项目
国家自然科学基金资助项目(39700167) （39700167）
