高等学校化学学报2012，Vol.33Issue(4)：683-688,6.DOI:10.3969/j.issn.0251-0790.2012.04.008
卤代荧光素荧光猝灭法测定肾上腺色腙
Fluorescence Quenching Method for the Determination of Carbazochromum with Halide Fluorescein Dyes
摘要
Abstract
In pH = 6. 6 Britton-Robinson(BR) buffer medium, a 1 : 2 (molar ratio) compound of diehlorofluo-rescein( DCF) , dibromofluorescein ( DBF) or diiodofluorescein ( DIF ) with carbazochromum ( CBZC ) was formed by electrostatic attraction, aromatic stacking interaction and van der Waals' force, which led to fluorescence quenching of above halide fluorescein dyes, and the maximum quenching wavelength were located at 533 nm(DCF) , 536 nm(DBF) and 560 nm(DIF) , respectively. Fluorescence quenching value(AF) is proportional to the concentration of CBZC in a certain range. Fluorescence quenching method for the determination of CBZC has high sensitivity, detection limits are 3. 3 ng/ml,( CBZC-DCF system) , 5. 7 ng/mL(CBZC-DBF system) and 129.6 ng/mL( CBZC-DIF system) , respectively. The effect of coexisting substances, the optimum conditions of the reaction and influencing factors were investigated. The results show this method has good selectivity. This method was successfully applied to the quick determination of CBZC in serum and urine samples. Judging from the effect of temperature, fluorescence lifetime determination and the Stern-Volmer plots, the quenching of fluorescence is a static quenching process.关键词
二氯荧光素/二溴荧光素/二碘荧光素/肾上腺色腙/荧光猝灭Key words
Dichlorofluorcscein/ Dibromofluorcscein/ Diiodofluorescein/ Carbazochromum/ Fluorescence quenching分类
化学化工引用本文复制引用
甘晓娟,刘绍璞,刘忠芳,胡小莉..卤代荧光素荧光猝灭法测定肾上腺色腙[J].高等学校化学学报,2012,33(4):683-688,6.基金项目
国家自然科学基金(批准号:20875078)资助. （批准号:20875078）
