高等学校化学学报2012，Vol.33Issue(5)：942-947,6.DOI:10.3969/j.issn.0251-0790.2012.05.014
超分子有序聚集凝固液相微萃取及其对五味子中木脂素类化合物的测定及质量评价
Solidification of Superamolecule Ordering Assembly Liquid Phase Microextraction and Its Application for the Determination and Evaluation for Lignans Quality in Schisandra Chinensis( Turcz.) Baill
摘要
Abstract
The extraction mechanism of solidification of superamolecule ordering assembly liquid phase micro-extraction(SSMOALPME) was proposed based on the change in the UV spectra of lignan compounds before and after the solidification of floating organic drop liquid phase microextraction(SFODLPME). SSMOALPME coupled with high performance liquid chromatography( HPLC) was introduced for the determination and evaluation of five low abundance lignan compounds in Schisandra chinensis (Turcz. ) Baill from different origins. Under the optimized parameters, the linear ranges of schisandrol A, schisantherin A, schizandrin A, schizan-drin B and schizandrin C were 2.48xl0-3-6. 21, 2. 27xl0-3-28. 5, 2. 31×l0-3-28. 8, 2.27×l0-3-5. 69 and 1.05×10-3-5.25 μg/mL, the limits of detection(LODs) were 0.4, 0.4, 0.4, 0.08 and 0.08 ng/mL, respectively, the intra and inter RSDs were less than 9. 7% , the mean recoveries of analytes in traditional Chinese medicines(TCMs) were between 91.9% and 104. 7% , and the enrichment factors( EFs) were in the range of 39-529 for SFODLPME. There was no significant difference in results determined lignan compounds in Schisandra chinensis (Turcz.) Baill by this method and Pharmacopoeia method (P = 95%). SSMOALPME-HPLC provide the theoretic foundation and experimental evidence for reflecting bioactive component characteristics of multicomponent, multi-targets, synergistic effect, and establishing scientific quality control methods in TCMs.关键词
漂浮有机液滴凝固液相微萃取/超分子有序聚集/富集倍数/五味子/木脂素Key words
Solidification of floating organic drop liquid phase microextraction ( SFODLPME) / Superamolecule ordering assembly/ Enrichment factor/ Schisandra chinensis (Turcz. ) Baill/ Lignan分类
化学化工引用本文复制引用
薛雪,张红芬,白小红,岳媛..超分子有序聚集凝固液相微萃取及其对五味子中木脂素类化合物的测定及质量评价[J].高等学校化学学报,2012,33(5):942-947,6.基金项目
国家自然科学基金(批准号:81041084)、山西省自然科学基金(批准号:2011011035-2)和山西省高等学校优秀创新培育团队项目资助. （批准号:81041084）
