肝胆胰外科杂志2012，Vol.24Issue(3)：228-231,4.
TNF-α McAb对急性失血性休克大鼠肝脏的作用
The effect of tumor necrosis factor- α monoclonal antibody in liver of rats on acute hemorrhagicshock
邱兆磊 1王振杰 1郑传明 1程兴望 1吴华2
作者信息
- 1. 蚌埠医学院第一附属医院急诊外科,安徽蚌埠233004
- 2. 蚌埠医学院第一附属医院肝胆外科,安徽蚌埠233004
- 折叠
摘要
Abstract
Objective To probe the role of tumor necrosis factor a (TNF-α) in liver injury of rats on acute hemorrhagic shock and the protective effect of tumor necrosis factor α monoclonal antibody(TNF- α Mr.Ab). Methods Twenty-four male Sprague-Dawley rats were randomly divided into three groups: control group (A), shock+lactated Ringer's solution resuscitation group(B) and shock+TNF- α McAb resuscitation group (C). Through the femoral artery bleeding, animal models of acute hemorrhagic shock were established. B group with lactated Ringer's solution resuscitation, C group with lactated Ringer's solution resuscitation containing TNF- α McAh(3mg/kg), while the A group under the same conditions without loss of blood. The levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and TNF- α were detected in each group. The contents of malondialdehyde (MDA) and superoxide dismutase (SOD) in liver tissue were measured. The pathological changes of liver tissue were observed under light and electron microscopy. Results The serum levels of ALT, AST, TNF- α and the content of MDA in liver tissue increased in B and C group compared with A group, while the content of SOD decreased. The serum levels of ALT ( 343.63 ± 35.61 ) U/L, AST ( 748.75 ± 49.76 ) U/L, TNF- α ( 99.38 ± 13.16 ) pg/mL and the content of MDA( 26.33 ± 1.30) nmol/mgProt in liver tissue in C group were much lower than those in B group, but the content of SOD (510.14 ± 47.44) U/mg Prot was increased. The changes of liver tissue histopathnlngy were minor in C group. Conclusion TNF- α may be one of an important factor in liver injury induced by acute hemorrhagic shock. Use of TNF- α McAb resuscitation can reduce liver injury and has a protective effect in rats with acute hemorrhagic shock. 关键词
肝损伤/肿瘤坏死因子-α/肿瘤坏死因子-α单克隆抗体/急性失血性休克/大鼠Key words
liver injury/ tumor necrosis factor α (TNF- α)/ TNF- α monoclonal antibody (TNF- α McAb)/acute hemorrhagic shock/ rats分类
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邱兆磊,王振杰,郑传明,程兴望,吴华..TNF-α McAb对急性失血性休克大鼠肝脏的作用[J].肝胆胰外科杂志,2012,24(3):228-231,4.
