国际医药卫生导报2012，Vol.18Issue(10)：1403-1406,4.DOI:10.3760/cma.j.issn.1007-1245.2012.10.013
改良冰冻血小板制备方法的评价
Evaluation on the improved preparation methods for the frozen platelet
摘要
Abstract
Objective To retrospectively analyze the situation about the frozen platelet preparation and melting in Dongguan city central blood station from 2008 to 2011,to evaluate the improved preparation method for the frozen platelet and the influence of irreversible aggregation after melting.Methods Statistical situation of the frozen platelet after melting since we used 75％ DMSO as cryoprotectants to make the frozen platelet from 2010 to 2011 was carried out,and as the experimental group.Statistical situation of the frozen platelet after melting since we used DMSO stock solution as cryoprotectants to make the frozen platelet from 2008 to 2009 was also conducted,and as the control group.The experimental group was frozen for one hour in the -50℃ Instant Freezer after adding 75％ DMSO solution,then transferred to -80 ℃ refrigerator,that was the improved method.The control group was frozen in -80 ℃ refrigerator after added DMSO stock solution,which was the classical method.If we visually found playlet irreversible aggregation after melting then the judgment was unqualified.Resula The rate of irreversible platelet aggregation was 0.37％(5/1352) in the experimental group after melting and there was no untoward effect in clineal applieation,the rate of irreversible platelet aggregation was 7.94％(98/1234).Between the two groups of irreversible platelet aggregation rate there was very significant difference (P ＜ 0.01).Conclusions With 75％ DMSO solution instead of DMSO stock solution as cryoprotectant added to the platelets suspension,-50℃ quickfrozen I hour and then transferred to -80 ℃ refrigerator,which is a very good method to make frozen platelet with very low rate of the irreversible platelet aggregation.We think it is worthy of wide popularizalion and application.关键词
冰冻血小板/二甲基亚砜/速冻Key words
Frozen platelet/DMSO/Quick-frozen引用本文复制引用
叶柱江,何子毅,陈金凤,黄志森,邓穗英..改良冰冻血小板制备方法的评价[J].国际医药卫生导报,2012,18(10):1403-1406,4.基金项目
东莞市科技项目(201110515000193) （201110515000193）
