果树学报2012，Vol.29Issue(3)：382-386,5.
湖北省十堰地区野生板栗cpSSR遗传多样性研究
Diversity of wild chestnut chloroplast DNA SSRs in Shiyan
摘要
Abstract
In order to study the genetic background of the wild Chinese chestnut resources in Shennongjia, four pairs of chloroplast simple-sequence repeats （cpSSRs） were used to investigate the population genet- ic structure and the distribution of chloroplast haplotypic variation in the wild populations of Chinese chestnuts collected from Shiyan prefecture of Hubei province. Four cpSSR presented 10 variations in the tested plants. Average number of alleles （A） and effective number of alleles （Ne） were 2.5 and 1.696, re- spectively. Average PIC was 0.312. Five haplotypes were found among the 53 tested individuals. 66% of all the individuals shared the same haplotype, while one sample in the Fangxian population showed a specific haplotype. With 0.4026 and 0.3794 of heterozygosity, the Danjiangkou and Fangxian populations showed a relatively higher haplotype diversity than other two. AMOVA analysis showed that 83% of the total cpSSR variation was attributable to differences among populations, and 17% to differences within popula- tions. The results indicated that high diversity of the wild C. rnollissima in Shiyan prefecture existed and cpSSR haplotype analysis would be useful for identifying the regional distribution of wild Chinese chestnut.关键词
板栗/叶绿体微卫星/单倍型/遗传差异Key words
Castanea mollissima L./cpSSR/Haplotype/Genetic diversity分类
农业科技引用本文复制引用
程丽莉,封海东,饶群,吴伟,周明,胡广隆,黄武刚..湖北省十堰地区野生板栗cpSSR遗传多样性研究[J].果树学报,2012,29(3):382-386,5.基金项目
国家林业局公益行业专项 （）
“林果种质资源创新”项目 （）
