广东药学院学报2012，Vol.28Issue(2)：149-152,4.DOI:10.3969/j.issn.1006-8783.2012.02.011
大孔吸附树脂分离纯化泽泻中4种三萜类化合物的工艺研究
The isolation and purification technology of four triterpenoids from Alismatis Rhizoma by macroporous resin
摘要
Abstract
Objective To study on the isolation and purification technology of four triterpenes from Alismatis Rhizoma by macroporous resin. Methods The optimization of the isolation was performed by static and dynamic adsorption parameters with 24-acetyl alisol A.alisol B,23-acetyl alisol B and ll-deoxy-23- acetyl alisol B in Alismatis Rhizoma as indicators. Results D-4020 macroporous resin showed the best performance for simultaneous separation and purification of 4 triterpenoids. Optimal parameters were as follows- the crude drug concentration of 0.50 g/mL, resin and the (g: g) with 1:1,first eluting with distilled water,then 50% ethanol eluting for 5 BV,and 90% ethanol for 8 BV with elution flow rate of 2 BV/h. 90% ethanol eluting to collect and dry to obtain the solid samples with four triterpenoids' content of 18. 68%. Conclusion D-4020 macroporous resin in the process conditions may be suitable for the simultaneous purification of the triterpenoids.关键词
大孔吸附树脂/泽泻/三萜类成分/分离纯化Key words
macroporous resin/ Alismatis Rhizoma/ triterpene/ purification分类
中医学引用本文复制引用
邱丽莉,吴水生,许文,刘海元,王思坚,罗永东..大孔吸附树脂分离纯化泽泻中4种三萜类化合物的工艺研究[J].广东药学院学报,2012,28(2):149-152,4.基金项目
福建省科技厅重大科技专项项目(2009YZ0001-1-5) （2009YZ0001-1-5）
