国际妇产科学杂志2012，Vol.39Issue(2)：199-201,3.
华蟾素与子宫内膜癌HHUA细胞侵袭性生长的实验研究
Effect of Cinobufagin on the Invasive Growth of Endometrial Carcinoma HHUA Cells
王绍光 1姜学强1
作者信息
- 1. 264000 山东省烟台市,青岛大学医学院附属烟台毓璜顶医院妇产科
- 折叠
摘要
Abstract
Objective:To investigate the effect of cinobufagin on the invasive growth of the endometrial carcinoma HHUA cells cultured in vitro. Methods:Endometrial carcinoma HHUA cells were cultured in vitro;after the action of cinobufagin with different concentration, MTT method was used to detect the proliferation of HHUA cells, the cellular apop-tosis was tested by flow cytometry,the expression of CD44s was measured by RT-PCR,the ability of invasion was compared with transwell chamber model. Results:Endometrial carcinoma HHUA cells expressed CD44s mRNA of (1.31±0.25) .number of invasive cell was 60.50±2.52;0.25 g/L cinobufagin didn't affect CD44s and invasive cell(P>0.05); after the action of 2.5 g/L cinobufagin,the expression of CD44s was 1.02±0.23, number of invasive cell was (30.50±3.95),these deta were different from the control group of endometrial carcinoma HHUA cells significantly (P<0.05); after the action of 25 g/L cinobufagin,the expression of CD44s was 0.97±0.39,number of invasive cell was 22.50±4.22,these data were different from the control group of endometrial carcinoma HHUA cells significantly (P<0.05) ;the action of 25 g/L cinobufagin was similar to that of 0.25 g/L cinobufagin(P>0.05). Conclusions:2.5 g/L cinobufagin inhibit the expression of CD44s mRNA and inhibit the invasive growth of the endometrial carcinoma HHUA cells.关键词
华蟾蜍毒素/子宫内膜肿瘤/基因/细胞凋亡/治疗Key words
Cinobufagin/ Endometrial neoplasms/ Genes/ Apoptosis/ Therapy引用本文复制引用
王绍光,姜学强..华蟾素与子宫内膜癌HHUA细胞侵袭性生长的实验研究[J].国际妇产科学杂志,2012,39(2):199-201,3.
