广西科学院学报2012，Vol.28Issue(2)：117-119,3.
枯草芽孢杆菌高效转化及其转化子验证方法
Method for Enhancing the Transformation Efficiency in Bacillus subtilis
摘要
Abstract
In order to study an effective method for transformation, Bacillus subtilis WB600 competent cells, transformed with pre-built recombinant plasmids, were precultured with different period of time and smeared to the plates containing antibiotics. In order to construct a fast and effective method for transformants verification, WB600 competent cells were transformed with vector and foreign DNA, preincubated with the optimal time, and smeared to the plates containing antibiotics. The transformants were verified with PCR and double restriction enzyme digestion.关键词
枯草芽孢杆菌/转化方法/转化率/感受态细胞Key words
Bacillus sub tilis/transformation approach/transformation efficiency/competent cell分类
生物科学引用本文复制引用
陆雁,王青艳,朱绮霞,秦艳,申乃坤,廖思明,谢能中,黄日波..枯草芽孢杆菌高效转化及其转化子验证方法[J].广西科学院学报,2012,28(2):117-119,3.基金项目
广西自然科学基金重点项目（2010GXNSFD013030）、广西培养新世纪学术和技术带头人专项资金项目、广西科学研究与技术开发课题项目（桂科攻11107008-4）资助. （）
