南方农业学报2012，Vol.43Issue(3)：267-271,5.DOI:10.3969/j:issn.2095-1191.2012.03.267
甘蓝型油菜Ogura胞质雄性不育系及恢复系聚类分析
Cluster analysis of the Ogura male sterile lines and fertility restorer lines in Brassica napus using SSR markers
摘要
Abstract
[Objective]The present study was conducted to analyze genetic diversity of Ogura male sterile line and restorer line to provide reference for breeding strong heterosis hybrids of Brassica napus using Ogura cytoplasm sterility. [Method ]Seventy six pairs of SSR primers distributed on linkage map were used for variance analysis and cluster analysis among 79 Ogura male sterile line and restorer line of Brassica napus. [Result]Among 76 pairs of SSR primers, 38 were selected to amplify 123 clear bands ranging from 100?00 bp in 79 accessions, and 89 polymorphic loci were obtained. Genetic similarity ranged between 0.52-0.99. Seventy梟ine B. napus lines were clustered into five groups of A, B, C, D and E at coefficient of 0.68. About 92.4% of total lines were classified into groups A and B, and 72.1% of the male sterile lines were classified into group A, 72.8% of the restorer lines were classified into group B. Croups A and B could also be divided into A1, A2, A3, A4, A5, A6, A7 and B1, B2, B3, B4 sub-groups at coefficient of 0.74, respectively. [Conclusion]SSR marker can be used for identifying classification Ogura male sterile line and restorer line.关键词
甘蓝型油菜/Ogura细胞质雄性不育/SSR标记/聚类分析Key words
Brassica napus/ Ogura cytoplasmic male sterility/ SSR marker/ cluster analysis分类
农业科技引用本文复制引用
罗延青,俎峰,周丕才,张国建,王敬乔,董云松..甘蓝型油菜Ogura胞质雄性不育系及恢复系聚类分析[J].南方农业学报,2012,43(3):267-271,5.基金项目
国家863计划项目(201110104) （201110104）
云南省现代农业油菜产业技术体系(云农科[2011]74号) （云农科[2011]74号）
