南方农业学报2012，Vol.43Issue(3)：286-289,4.DOI:10.3969/j:issn.2095-1191.2012.03.286
6-BA和2,4-D对黄独带芽茎段生长发育的影响
Effects of 6-BA and 2, 4-D on growth and development of Dioscorea bulbifera L.budded stems
摘要
Abstract
[Objective]The effects of 6-BA and 2,4-D on growth and development of Dioscorea bulbifera L budded stems was studied in order to utilize the technique in large scale production of Dioscorea bulbifera L. piantlets. (Method] Using plant tissue culture methods, D. bulbifera L. budded stems ( 0.5-1.5 cm ) were inoculated into liquid culture media in single factor experiment. [Result]MS medium was best for bud multiplication, rooting, and increase in shoot diameter. The best medium for growth of regenerated buds and increase in bud diameter and shoot length of D. bulbifera L. were identified as MS+6-BA 1 mg/L+2,4-D 0.5 mg/L,MS+6-BA 2 mg/L+2,4-D 0.1 mg/L and MS+6-BA 2 mg/L+2,4-D 0.1 mg/L, respectively. [Conclusion]The hormone combination 6-BA and 2,4-D could be used for growth of regenerated buds and increase in bud diameter and shoot length of D. bulbifera L.关键词
黄独/带芽茎段/6-BA/2,4-D/生长发育Key words
Dioscorea bulbifera L./ steins with a bud/ 6-BA/ 2,4-D/ growth and development分类
农业科技引用本文复制引用
洪森荣,程细云..6-BA和2,4-D对黄独带芽茎段生长发育的影响[J].南方农业学报,2012,43(3):286-289,4.基金项目
江西省教育厅2011年度一般科技项目(GJJ11610) （GJJ11610）
上饶师范学院植物学创新团队2011年校设招标课题资助项目(Z201101) （Z201101）
上饶师范学院科研基金资助项目(SR1013) （SR1013）
