广西猪源H9N2亚型流感病毒全基因组序列分析OA北大核心CSCDCSTPCD

[Objective]The study was undertaken to investigate the sources and variation of H9N2 influenza virus from swine in Guangxi and to provide a reference for its prevention and control. [Method]The genomic DNA of influenza virus strain A/SW/GX/S11/05(H9N2), was isolated, amplified, cloned and sequenced. [Result]The length of HA, NA, NP, M, NS, PB1, PB2 and PA genes was 1683, 1401, 1497, 1027, 890, 2233, 2280 and 2151 bp, respectively, and the length of deduced amino acids was 561, 467, 499, 349, 338, 744, 760 and 717 aa. The results revealed that nucleotide and deduced amino acids of the strain had 96.2-100.0% and 98.5-99.6% homology with strain A/Bird/GX/62/05, respectively. The systemic evolutionary analysis also proved that the genetic relationship between strain SW/GX/S11/05 and A/Bird/GX/62/05 was the closest. [Conclusion]The H9N2 subtype influenza virus from swine (collected in Guangxi) belonged to Beijing subspecies of Eurasian pedigree, and could infect to various hosts in a certain period. Consequently, trans-species and genus transmission should be taken into account in terms of prevention and control of the influenza viruses in future.