茶树油调控树突状细胞的表型和功能OA北大核心CSTPCD
Tea Tree (Melaleuca alternifolia) Oil induced Phenotypic and Functional Maturation of Murine Bone Marrow-derived Dendritic Cells
本文主要探讨了茶树油对树突状细胞表型和功能的影响.采用茶树油(0.005μL/mL)干预未成熟的树突状细胞48h后,流式细胞仪检测细胞表型,MTT法检测刺激淋巴细胞增殖能力.结果发现经茶树油干预后,以CD11c设门,树突状细胞高表达H-2Db(48.20％),I-Ab(6.60％)和CD86 (41.00％),和空白对照组比较分别达到1.28倍,3.40倍和1.28倍的增长.混合淋巴培养实验发现茶树油能够增强DC刺激淋巴细胞增殖的能力,降低树突状细胞的吞噬功能.初步表明茶树油可以促进树突状细胞的表型和功能成熟.
The effect of tea tree oil on the phenotypic and functional maturation of dendritic cell was investigated in vitro. Dendritic cells treated with tea tree oil (0.005 μL/mL), were enlarged, suspended, and displayed typical stellate and aggregates like cells treated with LPS (positive controls). The levels of surface marker expression were monitored throughout the culture by flow cytometry using CD1 1c-PEcy7, I-Ab-FITC, CD86-FITC, and H-2Db-PE. Dendritic cells treated with tea bee oil expressed higher level of H-2Db (48.20%), costimulatory molecule I-Ab (6.60%) and CD86 (41.00%) with the cells gated by CD11c, being of 1.28,3.40 and 1.28-fold higher for H-2Db, I-Ab and CD86, respectiely compared with untreated cells. It was also found that tea tree oil could enhance the dendritic cell to induce proliferation of T lymphocyte. And it was reduced the endocytic activity of dendritic cell by tea tree oil. These results showed that tea tree oil had significant immunoenhancing activity by inducing the phenotypic and functional maturation of dendritic cells.
唐永富;李积华;陈家翠;林丽静;韩志萍;曹玉坡
中国热带农业科学院农产品加工研究所/农业部热带作物产品加工重点开放实验室,广东湛江524001国家重要热带作物工程技术研究中心,海南海口571101中国热带农业科学院农产品加工研究所/农业部热带作物产品加工重点开放实验室,广东湛江524001国家重要热带作物工程技术研究中心,海南海口571101中国热带农业科学院农产品加工研究所/农业部热带作物产品加工重点开放实验室,广东湛江524001中国热带农业科学院农产品加工研究所/农业部热带作物产品加工重点开放实验室,广东湛江524001
茶树油树突状细胞表型吞噬活性
tea tree oil dendritic cell phenotypic endocytic activity
《现代食品科技》 2012 (6)
606-609,626,5
海南省自然科学基金(809025、311067)中央级公益性科研院所基本科研业务费专项资金资助(ITBBKF093)
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