乙醇/硫酸铵双水相体系从丹参粗提液中分离丹酚酸BOA北大核心CSCDCSTPCD
A new aqueous two-phase system of ethanol/ammonium sulphate for recovery of lithospermic acid B from extract of Salvia miltiorrhiza Bge
采用中心复合设计的响应曲面法优化了乙醇/硫酸铵双水相体系从丹参粗提液中分离丹酚酸B的工艺参数,并对放大效果进行了对比研究.结果表明,在确定pH2.0和30℃的条件下,当硫酸铵和乙醇质量分数分别为20％和29％时,丹酚酸B的分配系数最大为58.7,回收率为97.3％.上相加乙醇脱除硫酸铵,减压浓缩、干燥,丹酚酸B的纯度为57.4％.与小试相比,放大40倍后,分配系数(59.3)、回收率(98.2％)和纯度(58.6％)均无显著差异(p＜0.05).…More>>
The separation of lithospermic acid B (LAB) from crude extract of Radix Salivae miltiorrhizae by aqueous two-phase systems was studied. According to the partition coefficient and the yield of LAB, various ethanol and (NH4 )2 SO4 systems were used and examined with response surface methodology to find the optimal condition. The results showed that the optimum phase composition was 20% (mass) of (NH4)2SO4 and 29% (mass) of ethanol for crude extraction at pH 2.…More>>
郭永学;王立红;张大勇;周丽莉;修志龙
沈阳药科大学制药工程学院,辽宁沈阳110016沈阳药科大学制药工程学院,辽宁沈阳110016沈阳药科大学制药工程学院,辽宁沈阳110016沈阳药科大学制药工程学院,辽宁沈阳110016大连理工大学生命科学与技术学院,辽宁大连116024
化学化工
丹酚酸B双水相萃取乙醇/硫酸铵响应面分析
lithospermic acid B aqueous two-phase extraction ethanol/ammonium sulphate response surface methodology
《化工学报》 2012 (6)
1773-1779,7