NTG诱变选育抗逆性蜡蚧轮枝茵及其生物学特性测定OA北大核心CSCDCSTPCD
Screening of Resistant Mutants by NTG Mutation and Study on Biological Characteristics of Verticillium lecanii
采用NTG对杀虫真菌蜡蚧轮枝菌进行诱变选育,期望获得抗逆性显著增强的菌株,以提高其在田间应用的适应性.以VL-18为出发菌株,用4mg/mL的NTG诱变处理120min,得到耐热性明显提高的诱变菌株NTG-2.抗逆性研究结果表明,NTG-2菌株经40℃水浴处理20min,孢子萌发率比原始菌株提高了251.4％,0.5min内的紫外耐受力提高了145.7％,酸碱耐受力无显著变化.毒力测定结果显示,第5天突变菌株NTG-2对蚜虫的致死中浓度(LC60…查看全部>>
An insecticidal fungus Verticillium lecanii was mutagenized to screen better field-application ones by NTG mutation methods, which were expected to have great stress tolerance in the field environment. The original strain VL-18 was treated by 4 mg/mL of NTG for 120 min,and then a mutant NTG-2 with higher thermostability was obtained. The stress tolerance of NTG-2 was studied and the results showed that when exposed at 40? for 20 n::;the conidiospore ge…查看全部>>
谷祖敏;谢阳;韩金栋;李斐;张丽莹
沈阳农业大学植物保护学院,辽宁省生物农药工程技术研究中心,辽宁沈阳110866沈阳农业大学植物保护学院,辽宁省生物农药工程技术研究中心,辽宁沈阳110866沈阳农业大学植物保护学院,辽宁省生物农药工程技术研究中心,辽宁沈阳110866沈阳农业大学植物保护学院,辽宁省生物农药工程技术研究中心,辽宁沈阳110866沈阳农业大学植物保护学院,辽宁省生物农药工程技术研究中心,辽宁沈阳110866
农业科技
杀虫真菌蜡蚧轮枝茵NTG诱变抗逆性生物学特性
insecticidal fungus Verticillium lecanii NTG mutation stress resistance biological characteristics
《河南农业科学》 2012 (5)
83-86,4
辽宁省教育厅项目(L2010489)
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