海洋科学2012，Vol.36Issue(5)：65-70,6.
低聚壳聚糖的美拉德反应及其衍生物的抗氧化性能研究
The Maillard reaction of chitosan oligosaccharide and the antioxidative activities of its derivatives
摘要
Abstract
Three kinds of chitosan oligosaccharide derivatives prepared through Maillard reaction by heating chi- tosan oligosaccharide for 8, 16 and 24 hours were named CC-8, CC-16 and CC-24, respectively. The pH, absorb- ance and fluorescence were determined during the reaction. Chitosan oligosaccharide derivatives were characterized by Fourier transform infrared （FTIR） spectroscopy and their molecular weights were determined by Gel permeation chromatograph （GPC）. Their antioxidative activity was investigated by employing various established in vitro sys- tems, such as superoxide anion, 1-diphenyl-2-picrylhydrazyl （DPPH） radicals scavenging, and their reducing capacity was also studied. The results showed that the pH value of the reaction system decreased during Maillard reaction. The UV-Vis absorbance increased significantly at 280 nm and this increase slowed down after 16 hr. The fluorescence intensity significantly increased with Ex at 343 nm and Em at 420 nm and also slowed down after 16 hr. All the three derivatives kept the characteristic absorption peak of chitosan oligosaccharide. Their anti- oxidative and reducing capacities were both much stronger than those of the chitosan oligosaccharide and CC-16 has the strongest antioxidative activity.关键词
低聚壳聚糖/美拉德反应/抗氧化性能Key words
chitosan oligosaccharide/Maillard reaction/antioxidative activity分类
生物科学引用本文复制引用
孙涛,朱云,王燕萍,谢晶,薛斌..低聚壳聚糖的美拉德反应及其衍生物的抗氧化性能研究[J].海洋科学,2012,36(5):65-70,6.基金项目
“十二五”国家支撑计划项目 （）
上海市科委工程中心建设项目 （）
