基础医学与临床2012，Vol.32Issue(5)：520-524,5.
骨髓间充质干细胞对HT-29细胞所形成的裸鼠肿瘤的影响
The effects of bone marrow derived mesenchymal stem cells on the tumor development in nude mice from HT-29 cells
摘要
Abstract
Objective To study the effects of bone marrow derived mesenchymal stem cells on the growth and differentiation of the tumor developed from HT-29 cells. Methods In vivo, nude mice were divided into 3 groups-HT-29 group, BMSC group and HT-29 + BMSC group, then cell suspensions were subcutaneously injected into nude mice. Tumors were removed after 7 weeks. The volumes and weights of the tumors were measured and HE staining was performed. The expression of an epithelia marker-E-CADHERIN was detected using RT-PCR and immunohistochemistry staining. In vitro, HT-29 cells were co-cultured with BMSCs, the morphology of HT-29 cells was observed, and the expression of EMT related genes were detected using RT-PCR. Results In vivo, no tumor was observed in the BMSC group, and no significant difference was found in the volume and weight of tumors between the HT-29 + BMSC group and the HT-29 group. But the tumor cells in the HT-29 + BMSC group showed poorly differentiated and lower level expressed of E-CADHERIN compared with the HT-29 group. In vitro, after coculturing with BMSCs, morphological changes of HT-29 cells were observed and were consistent with the expression of EMT related genes. Conclusions The differentiation degree of HT-29 cells is correlated to EMT induced by BMSCs microenvironment.关键词
骨髓间充质干细胞/HT-29细胞/肿瘤生长和分化/共培养/EMT/微环境Key words
bone marrow derived mesenchymal stem cell/ HT-29 cell/ tumor growth and differentiation/ co-culture/ EMT/ microenvironment分类
医药卫生引用本文复制引用
巩福星,焦虎,董平,严笠,尹艳花,肖苒..骨髓间充质干细胞对HT-29细胞所形成的裸鼠肿瘤的影响[J].基础医学与临床,2012,32(5):520-524,5.基金项目
国家自然科学基金(30871433,31071305) （30871433,31071305）
