基础医学与临床2012，Vol.32Issue(7)：734-738,5.
Genistein后处理通过激活eNOS保护缺血后海马CA1区神经元
Genistein postconditioning protects neurons against ischemic injury through eNOS activation in hippocampal CA1 region
摘要
Abstract
Objective To investigate the neuroprotective role of Genistein postconditioning (GPC) against cerebral ischemic injury and its effects on the level of eNOS phosphorylation in hippocampal CA1 region of rats. Methods The rats weTe subjected to global cerebral ischemia by four-vessel occlusion and randomly divided into five groups, sham, ischemia/reperfusion (I/R), GPC (intravenously through the tail vein) , L-NAME (an inhibitor of NOS, icv. ) and vehicle (I/R + DMSO, I/R + Genistein + NaCl) groups. The protein level of p-eNOS and eNOS was detected by Western blotting. Additionally, NeuN staining and TUNEL analysis was used to detect the survival neurons and apoptotic neurons of hippocampal CA1 region, respectively by Laser Scanning Confocal Microscope. Results Compared with I/R groups, in GPC groups p-eNOS levels at 30 min or 3 d of reperfusion significantly in-creased (P < 0. 001), while eNOS protein expression in all time points had no obviously change. CPC groups compared with I/R groups, the number of surviving neurons of hippocampal CA1 region significantly increased (213. 0 ±21. 0 vS 45. 0 + 15. 0, P <0. 05) and apoptotic neurons markedly reduced (29. 0 ±6. 0 vs 192. 0 ±31. 0, P <0. 05). Furthermore, L-NAME can not only significantly abate neuroprotection induced by genistein postcondi-tioning(P<0.05), but also effectively reduce the level of p-eNOS (1. 149 ±0.218 vs 1.583 ±0. 155, P < 0. 001). Conclusions GPC significantly prevents neuronal injury from global cerebral ischemia in the hippocampal CA1 region of rats. The mechanism might be explained by the increase of p-eNOS level.关键词
Genistein后处理/脑缺血/再灌注/p-eNOS/海马CA1区Key words
Genistein postconditioning/ cerebral ischemia/reperfusion/ p-eNOS/ hippocampus CA1 region分类
医药卫生引用本文复制引用
涂静宜,朱莹,周彩凤,张茜,杨方,王瑞敏..Genistein后处理通过激活eNOS保护缺血后海马CA1区神经元[J].基础医学与临床,2012,32(7):734-738,5.基金项目
国家自然科学基金(30970664,31171354) （30970664,31171354）
河北省教育厅自然科学计划项目(2008140) （2008140）
