基础医学与临床2012，Vol.32Issue(7)：744-750,7.
SD118-2对人HepG2细胞凋亡影响及机制探讨
The effects and mechanisms of SD118-2 on human HepG2 cells apoptosis
摘要
Abstract
Objective To study the effects of SD118-2 on human HepG2 cells proliferation, cell cycle and cell apoptosis. Methods The proliferation of cells was detected by MTT assay. The modality of cells was detected by the fluorescence microscopy with DAPI staining. The cell apoptosis, cell cycle and mitochondrial membrane potential were detected by the flow cytometer. The apoptosis associated with BCL-2,BAX,PARP,C-PARP was detected by Western blot. Results SD118-2 inhibited the proliferation of human HepG2 cells, decreased the mitochondrial membrane potential and induced cell apoptosis, blocked the cells in G2 stage, down-regulated the expression of BCL-2 and up-regulated expression of BAX,C-PARP at a time-dependent manner. At the same time, the effects of SD118-2 on normal human liver was very weak. Conclusions SD118-2 can inhibit the cell proliferation, induce cell apoptosis and block the cell in G2 stage of HepG2.关键词
SD118-2/人HepG2细胞/凋亡/线粒体膜电位Key words
SD118-2/ HepG2 cells/ apoptosis/ mitochondrial membrane potential分类
生物科学引用本文复制引用
戴焱焱,陈若华,黄才国..SD118-2对人HepG2细胞凋亡影响及机制探讨[J].基础医学与临床,2012,32(7):744-750,7.基金项目
国家自然科学基金973项目(2010CB833804) （2010CB833804）
国家自然科学基金863项目(2011AA09070110) （2011AA09070110）
