共转染沉默5-LOX/COX-2基因对胃癌细胞SGC-7901生长的影响OA北大核心CSCDCSTPCD
Effect of the 5-LOX/COX-2 gene silencing via cotransfection on the growth of SGC-7901 human gastric cancer cells
目的 观察沉默环氧合酶2(COX-2)基因和5-脂氧合酶(5-LOX)基因对胃癌细胞SGC-7901生长的影响.方法 分别构建5-LOX/COX-2基因的靶向短发夹RNA(shRNA)真核表达质粒,共同转染胃癌细胞SGC-7901.将细胞分为空白对照组、空质粒组、单独转染组(P-sh5-LOX或P-shCOX-2)及共同转染组(P-sh5-LOX+P-shCOX-2).分别采用RT-PCR及Western blotting检测COX-2/5-LO…查看全部>>
Objective To observe the effects of cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) silencing on the growth of SGC-7901 human gastric cancer cells. Methods The eukaryotic expression plasmids of targeted short hairpin RNA containing the S-LOX and COX-2 genes were constructed for the co-transfection of gastric cancer cells. The cells were divided into control group, plasmid group, alone transfection groups (P-shS-LOX and P-shCOX-2), and co-transfection gro…查看全部>>
刘代江;刘纯伦;张瑶;窦娟
400016重庆 重庆医科大学附属第一医院消化内科400016重庆 重庆医科大学附属第一医院消化内科400016重庆 重庆医科大学附属第一医院消化内科400016重庆 重庆医科大学附属第一医院消化内科
医药卫生
基因沉默胃肿瘤花生四烯酸盐5-脂氧合酶前列腺素内过氧化物合酶类
gene silencing stomach neoplasms arachidonate S-lipoxygenase prostaglandin-endoperoxide synthascs
《解放军医学杂志》 2012 (5)
444-448,5
重庆市卫生局资助课题(05-2-155)
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