江苏大学学报（医学版）2012，Vol.22Issue(4)：286-290,294,6.
OsmY缺失对伤寒沙门菌在高渗应激早期基因表达的影响
Effect of OsmY on gene expressional regulation of Salmonella enterica serovar Typhi at early stage of hyperosmotic stress
摘要
Abstract
Objective: To explore the effect of OsmY on gene expressional regulation of Salmonella en-tericasemvar Typhi (S. Typhi) at the early stage of hyperosmotic stress. Methods; The osmY gene deleted mutant of S. Typhi was generated through homologous recombination mediated by a suicide plasmid ; the gene expression profiles of the wild type strain and the osmY deleted mutant at early stage of hyperosmotic stress was investigated by genomic microarray assay ; qRT-PCR was performed to validate the results of mi -croarray assay. Results; The osmY deleted mutant of S. Typhi was prepared successfully ; analysis of ge-nomic microarray assay showed that 27 genes were up regulated and 128 genes were down regulated in the osmY deleted mutant at the early stage of hyperosmotic stress compared to the wild type strain . The results of qRT-PCR assay were consistent with the results of microarray assay . Conclusion; osmY of S. Typhi may be a regulator played an important role in regulating gene expression at early stage of hyperosmotic stress .关键词
伤寒沙门菌/OsmY/高渗应激/基因芯片Key words
Salmonella enterica serovar Typhi/ OsmY/ hyperosmotic stress / genomic microarray分类
医药卫生引用本文复制引用
孟彦辰,王菲,杜鸿,翁晓琴,张海方,生秀梅,徐顺高,黄新祥..OsmY缺失对伤寒沙门菌在高渗应激早期基因表达的影响[J].江苏大学学报（医学版）,2012,22(4):286-290,294,6.基金项目
江苏省高校自然科学基金资助项目(10KJB310001) （10KJB310001）
