军事医学2012，Vol.36Issue(7)：535-538,4.
F344大鼠4种饮用水饲养2年实验病理学观察
Pathological observation of F344 rats drinking four different types of water for two years
摘要
Abstract
Objective To investigate the possible effects of four different types of drinking water on the type and incidence of spontaneous lesions and mortality rates among F344 rats during a period of two years. Methods Three hundred and thirty-six the F344 rats at the SPF level, with males and females in half and at the age of eight weeks, were randomly divided into four groups:natural mineral water group, tap water, purified water group and mineral water group, respectively. Eight, sixteen, sixteen and forty-four rats in each group were sacrificed at 6, 12, 18 and 24 months after the start of the study. The mass of the heart, lung, liver, spleen, kidney, adrenal gland, thymus, brain, testes ( ovaries for the female ) and epididymis ( uterus for the female ) were recorded and the coefficients of the organs were calculated. Histopathological examination was conducted. The type and incidence of spontaneous lesions in each group were recorded and calculated. Results The type and incidence of spontaneous lesions, including non-neoplastic lesions and neoplastic lesions in each group, were compared. Non-proliferative lesions included murine progressive cardiomyopathy, lung bronchus-associated lymphoid tissue( BALT ), enlargement or atrophy of tubules, proteinaceous cast and calcification in the kidney, and genital tissue degeneration. The frequently found neoplastic lesions were Leydig cell tumor, adenoma in pituitary gland, large granu- lar lymphoma ( LGL ), subcutaneous fibroma, mammary adenoma and pheochromocytoma. No statistically significant was found between the four groups with regard to the type and incidence of lesions and mortality rates. Conclusion This study enriches the pathological data of the F344 rats and throwes light on the selection of appropriate drinking water for animals in GLP laboratories.关键词
大鼠,近交F344/饮用水/病理学/自发性病变Key words
rats inbred F344/ drinking water/ pathology/ spontaneous lesion分类
生物科学引用本文复制引用
尹纪业,董延生,袁本利,瞿文生,原野,王和枚,丁日高..F344大鼠4种饮用水饲养2年实验病理学观察[J].军事医学,2012,36(7):535-538,4.基金项目
国家重大新药创制科技专项资助项目(2008ZX09305-003 （）
2010ZXJ0900X-005) （）
