江西农业学报2012，Vol.24Issue(5)：73-75,3.
大活组织培养及再生体系建立的研究
Study on Tissue Culture and Establishment of Regeneration System in Vitro of Ranunculus sceleratus
摘要
Abstract
Taking the tender steins of Ranunculus sceleratus as explants, the tissue culture and establishment of regeneration system in vitro of Ranunculus sceleratus were studied. The optimum medium for callus inducement and subculture proliferation was MS + 6-BA 0.5 mg/L+2,4-D 2.0 mg/L: under the condition of illumination, the optimum medium for callus differentiation was MS + ZT 0.6 mg/L + NAA 0.1 mg/L. The optimum medium for the rooting of adventitious buds and subculture proliferation of test - tube seedlings was 1/4 MS + IAA 0.5 mg/L. The transplanting survival rate of the test - tube seedlings was 92.1% , and the stable planting survival rate was 96.8%.关键词
大活/组织培养/再生体系/建立Key words
Ranunculus sceleratus/ Tissue culture/ Regeneration system/ Establishment分类
农业科技引用本文复制引用
卢晓,刘爽,黄晓非,徐娜,姜长阳..大活组织培养及再生体系建立的研究[J].江西农业学报,2012,24(5):73-75,3.基金项目
辽宁省高等教育教学改革资助项目(20090304) （20090304）
辽宁师范大学教学改革资助项目(LSJG:20090108) （LSJG:20090108）
