江西农业学报2012，Vol.24Issue(5)：124-126,3.
重组Taq DNA聚合酶的表达和纯化鉴定
Expression, Purification and Identification of Recombinant Taq DNA Polymerase
刘钦松 1刘孟刚 1张丛丛 1张程 1许彤1
作者信息
- 1. 山东博奥克生物科技有限公司,山东聊城252000
- 折叠
摘要
Abstract
In order to express and purify Taq DNA polymerase, and detect its amplification performance, E. coli strain JM109 containing the gene of Taq DNA polymerase was activated, and the gene was expressed under the inducement of IPTG. The expressed protein was purified by AKTA protein purification system through passing Affi - Gel Blue Sepharose, Heparin Sepharose Fast Flow and Q - Sepharose Fast Flow in turn- Taking Taq enzyme imported from abroad as the standard, the enzyme activity was roughly determined through antitheses and SDS - PAGE analysis, and the product quality was verified finally. The results revealed that the prepared Taq DNA polymerase had the following good characteristics: high amplification efficiency, high purity, strong specificity, no nuclease pollution and stable activity. In conclusion, the recombinant Taq DNA polymerase was successfully expressed and purified, and its amplification performance could reach or exceed that of the similar products imported from abroad.关键词
Taq DNA聚合酶/纯化/扩增性能Key words
Taq DNA polymerase/ Purification/ Amplification performance分类
生物科学引用本文复制引用
刘钦松,刘孟刚,张丛丛,张程,许彤..重组Taq DNA聚合酶的表达和纯化鉴定[J].江西农业学报,2012,24(5):124-126,3.
