军医进修学院学报2012，Vol.33Issue(8)：873-875,3.DOI:10.3969/j.issn.1005-1139.2012.08.029
Bcl-2基因在人颈动脉粥样斑块中的表达
Expression of Bcl-2 gene in human carotid atheromatous plaques
摘要
Abstract
Objective To study the expression of Bcl-2 gene in human carotid atheromatous plaques and its influence on stability of atherosclerotic plaques. Methods Forty-two samples of carotid atheromatous plaques taken in endarterectomy were divided into stable group (n=19) and unstable group (n=23) according to their pathological typing. Eight samples of aortic artery and its branches taken from hepatic transplantation donors served as a control group. Expression of Bcl-2 was detected by immunohistochemistry (IHC) and in situ hybridization(ISH), respectively. Results IHC and ISH showed that the bcl-2 was expressed in unstable plaques from 20 and 9 patients, in stable plaques from 11 and 4 patients (P<0.05), and in vascular smooth muscle cells, macrophage cells, endothelial cells and foam cells. The number of Bcl-2 positive cells was 122 ± 28.84 and 258 ± 29.93 in unstable plaques, and 12.93 ± 4.66 and 11.32 ± 6.15 in stable plaques, respectively, with the Bcl-2 positive cells mainly concentrated in the shoulder area where inflammation cells accumulated. The expression level of Bcl-2 was significantly higher in unstable plaques than in stable plaques (P <0.01). No expression of Bcl-2 was found in control group. Conclusion The expression level of Bcl-2 is higher in unstable plaques than in stable plaques and is, thus, an anti-apoptotic and atheromatous plaques-maintaining factor of stability.关键词
颈动脉/动脉粥样硬化/细胞凋亡/Bcl-2基因Key words
carotid artery/ atherosclerosis/ apoptosis/ Bcl-2 gene分类
基础医学引用本文复制引用
门保忠,苏琴,周定标,石怀银,张笑明..Bcl-2基因在人颈动脉粥样斑块中的表达[J].军医进修学院学报,2012,33(8):873-875,3.基金项目
军队"十一五"科技攻关项目(2001BA703B16). （2001BA703B16）
