生物质化学工程2012，Vol.46Issue(3)：13-19,7.
绿液预处理玉米秸秆产纤维素酶的研究
The Research on Cellulase Production of Green Liquor Pretreated Corn Stover
摘要
Abstract
Abstract：The effect of green liquid pretreated corn stover on cellulase production of Trichoderma reesei was investigated. Inspecting the influence of pretreatment conditions on cellulase production of corn stover at different cooking temperature, total alkali charge and sulfidity by orthogonal experiment, we could conclude from range and variance analysis that the extent of impact to cellucase production of green liquor pretreated corn stover was in turn total alkali charge, cooking temperature and sultidity. The optimum filter paper activity （FPA） reached 2.6 IU/mL. FPA productions from corn stover oretreated bv cooking temperature140℃ and 170 ℃, total alkali charge 4 %, sulfidity 0, 20 %, 30 % and 40 %, were comprehensively compared and evaluated. It was found that the optimum conditions, i. e. , cooking temperature 140℃, total alkali charge 4 %, sulfidity 0, might ensure the value of FPA on and avoid the loss of materials. The results showed that green liquid pretreatment for corn stoverwas feasible for cellulase production of Trichoderma reesei. Taking corn stover pretreated by cooking temperature 140 ℃, total alkali charge 4% and sulfidity 0 as carbon sources whose concentration was 12 g/L, FPA and β-glucosidase activity（β-GA） were up to 2.5 IU/mL and 1.3 IU/mL respectively. The enzyme production period was 5 days.关键词
纤维素酶/里氏木霉/绿液预处理/玉米秸秆Key words
cellulase/Trichoderma reesei/green liquor pretreatment/corn stover分类
化学化工引用本文复制引用
赵士明,陆青山,谷峰,余世袁..绿液预处理玉米秸秆产纤维素酶的研究[J].生物质化学工程,2012,46(3):13-19,7.基金项目
国家林业局林业公益性行业科研专项 （）
江苏高校科技创新团队资助项目 （）
