摘要
Abstract
Purpose To investigate the clinical significance of approximated molecular subtypes of preoperative breast carcinoma on fine needle aspiration ( FNA ) cytology specimens. Methods The 42 female patients with primary breast carcinoma were aspirated by fine needle before operative. Six different subtypes, such as Luminal A, Luminal B, pure HER2-overexpressing, basal-like, basal-HER2 and null, were defined by five immunocytochemical markers, including estrogen receptor ( ER ), progesterone receptor ( PR ), human epidermal growth factor 2 ( HER2 ), epidermal growth factor receptor ( EGFR ) and Cytokeratin5/6 ( CK5/6 ) on cytology specimens. Corresponding tissue sections were detected for the same items with HE staining and immunohistochemical staining. The chi-square test was undertaken to establish the association between two methods. Results All 42 female patients with primary breast carcinoma were correctly diagnosed by FNA before operative if the histopathology results were regarded as gold standard. The sensitivity is 100% . A-mong the 42 cases, the positive rate of ER/PR, HER2, EGFR/CK5/6 were 52. 38%( 22/42 ), 42. 86%( 18/42 ), 19. 04%( 8/42 ), respectively in FNA smears, while 52. 38%( 22/42 ), 40. 48%( 17/42 ), 23. 81%( 10/42 ), respectively in tissue sections. There is no statistically significant difference between them ( P > 0. 05 ). Conclusions FNA is one of accurate and feasible measures to diagnose breast carcinoma before operation. Approximated molecular subtypes on FNA specimens before operation should be useful for pre-operatively realizing the tumor biological characters, guiding neoadjuvant chemotherapy and operation treatment.关键词
乳腺肿瘤/细针吸取/免疫细胞化学/分子分型Key words
breast neoplasms/ fine-needle aspiration/ immunocytochemistry/ molecular subtype分类
医药卫生
