环境与职业医学2012，Vol.29Issue(5)：290-293,4.
大鼠新生期苯并芘暴露对睾丸抗氧化酶活性和精子生成量的影响
Effects of Benzo(a)pyrene Exposure on Testicular Antioxidant Enzyme Activities in Neonatal SD Rats
摘要
Abstract
[ Objective ] To investigate the changes of antioxidant enzyme activities in testis of SD rats after neonatal exposure to 3, 4-benzo(a)pyrene. [ Methods ] The neonatal rats were treated with benzo(a)pyrene in com oil by gavages at doses of 0, 5, 10 and 25 mg/kg from postnatal day 1 to day 7. They were sacrificed at postnatal day 8, 35 and 90. Organ weights, daily sperm production, enzyme activities of superoxide dismutase (SOD) and catalase (CAT), and CYP1A1 gene expression levels of testis were determined at postnatal day 8, 35 and 90. [ Results ] CYP1AI gene expressions in exposed groups were induced in a dose-dependent manner at postnatal day 8, and that of the 10 and 25 mg/kg groups were significantly higher than that of the controls (P<0.05), but there was barely no CYP1AI gene expression in groups at postnatal day 35 and 90. At postnatal day 8, SOD enzyme activities in 25 mg/kg group were increased significantly than that in the controls (P<0.05); and CAT enzyme activities were increased with exposure doses; at postnatal day 35, the activities of both SOD and CAT decreased significantly than that of the controls (P<0.05); at postnatal day 90, there was significant difference in neither SOD nor CAT enzyme activities between exposure groups and controls; and the number of daily sperm production of 10 and 25mg/kg groups decreased significantly {P<0.05). [ Conclusion ] Neonatal exposure to benzo(a)pyrene altered activities of SOD and CAT enzymes in testis, and decreased daily sperm production.关键词
苯并芘/新生期暴露/精子计数/超氧化物歧化酶/过氧化氢酶/细胞色素P450氧化酶基因Key words
benzo(a)pyrene/ neonatal exposure/ sperm counts/ superoxide dismutase/ catalase/ CYP1A1分类
医药卫生引用本文复制引用
梁继仁,朱鸿雁,李翠珍,周志俊,吴庆..大鼠新生期苯并芘暴露对睾丸抗氧化酶活性和精子生成量的影响[J].环境与职业医学,2012,29(5):290-293,4.基金项目
国家自然科学基金资助项目(编号:20977019) （编号:20977019）
