环境与职业医学2012，Vol.29Issue(5)：294-298,5.
对氨基水杨酸钠对染锰大鼠基底核GABAAR及GAT-1表达的影响
Effect of PAS-Na on GABAAR and GAT-1 Protein and mRNA Levels in Basal Ganglia of Manganese-Exposed Rats
摘要
Abstract
[ Objective ] To explore the effect of sodium para-aminosalicylate (PAS-Na) on the expression of gamma-aminohutryic acid type A receptor (GABAaR) and gamma-aminobutryic acid transporter (GAT-1) in short-term or subchronic manganese (Mn)-exposed rats. [ Methods ] In the short-terra experiment, rats were divided into control, Mn-exposed, low-dose PAS-Na (L-PAS) and high-dose PAS-Na (H-PAS) groups, each subgroup of 10 rate were necropsied at the end of week 7 and 10. In the subchronic experiment, rats were divided into control, Mn-exposed, PAS-Na prevention (P-PAS), and L-PAS, M-PAS (middle-dose PAS-Na) and H-PAS groups, and were observed at week 4, 8, 12 and 18. The mRNA and protein expression of GABAaR and GAT-1 in rat basal ganglia were examined by real-time fluorescence polymerase chain reaction (RT-PCR) and Western blot (WB). [ Results ] In the short-term experiment, on observation week 7, GABAaR protein expression was significantly increased in Mn-exposed group (P<0.05); on observation week 10, GABAaR and GAT-1 mRNA expression were significantly decreased in Mn-exposed rats (P<0.05), and L-PAS and H-PAS treatment restored their GAT-1 mRNA expression (P<0.05). In the subchronic experiment, on obervation week 4, GABAaR mRNA expression was greatly increased in Mn-exposed rats (P<0.05); on observation week 8, GABAaR mRNA expression was greatly decreased in Mn-exposed rats vs controls (P<0.05) and increased in P-PAS group vs Mn-exposed rats (P<0.05); however, on obervation week 12, GABAaR protein expression was greatly increased in Mn-exposed rats vs controls (P<0.05) and decreased in P-PAS vs Mn-exposed rats (P<0.05); on observation week 18, GABAaR mRNA expression wasgreatly decreased in Mn-exposed rats vs controls (P<0.05), and CAT-1 mRNA expression was greatly increased in Mn-exposed rats vs controls (P<0.05) and decreased in H-PAS vs Mn-exposed rats (P< 0.05). [ Conclusion ] Short-term and suhohrnnic exposure of manganese exhibited obvious toxic effects in the expression of mRNA GABAaR and GAT-1 in basal ganglions of rats. PAS-Na played a preventive intervention role in the GABAaR mRNA or protein expression of subchronic Mn-exposed rals and a therapeutic intervention role in the GAT-1 mRNA expression.关键词
对氨基水杨酸钠/锰中毒/基底核/γ-氨基丁酸A受体/γ-氨基丁酸转运载体/实验治疗Key words
sodium para-aminosalicylate/ manganese poisoning/ basal ganglia/ Y-aminobutyric acid A receptor/Y-aminobutyric acid transporter/ experimental therapy分类
医药卫生引用本文复制引用
欧超燕,罗海兰,姜岳明,王禅,蒙浩洋,姜力,区仕燕,邓祥发..对氨基水杨酸钠对染锰大鼠基底核GABAAR及GAT-1表达的影响[J].环境与职业医学,2012,29(5):294-298,5.基金项目
国家自然科学基金资助项目(编号:81072320,30760210) （编号:81072320,30760210）
