南京林业大学学报(自然科学版)2012,Vol.36Issue(2):47-51,5.
油茶基因组微卫星特征分析
Characteristic analysis of microsatellites of Camellia spp.
摘要
Abstract
There were 11 344 microsatellites (SSRs) ranged from 1 to 6 bp detected by analyzing the DNA sequences covering about 10 % of genome Camellia. Among these SSRs, dinucleotide repeats were most abundant (27. 1 % ). For the composition of microsatellites, A/T repeats were the most frequent motifs in mononucleotide repeats, and AT/TA, AG/TC repeats were the most in dinucleotide repeats, while (AAN)n, (AAAN)ra and (AAAAN)re repeats were dominant in tri-, tetra- and pentanucleotide repeats, respectively. All the dominant repeat motifs for different type of SSRs were abundant in nucleotides A and T. In Camellia genome, microsatellites longer than 20bp accounted for about 11.7% of the detected SSRs. Repeats, repeat motif lengths were found negatively correlated with the variation rate except mononucleotide repeats, thus, the shorter repeats would have faster substitution rate.关键词
油茶/微卫星/重复单元/长度变异Key words
Camellia spp. / microsatellite/ repeat motif/ length variation分类
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史洁,尹佟明,管宏伟,戴晓港,陈金慧,施季森..油茶基因组微卫星特征分析[J].南京林业大学学报(自然科学版),2012,36(2):47-51,5.基金项目
国家林业局林业公益性行业科研专项项目(200904002) (200904002)