南京林业大学学报（自然科学版）2012，Vol.36Issue(2)：81-85,5.
3种杀虫剂对杨树羧酸酯酶的抑制效果
Inhibition effect of three insecticides on carboxylesterase from poplar
摘要
Abstract
The optimum reaction condition of carboxylesterase in poplar was studied using the biochemistry methods. The results indicated that the optimum enzyme dilution factor of carboxylesterase was 10, and the optimum substrate concentration was 3 × 10-4 mol/L, the optimum pH value was 7.0, and the optimum reaction time was 15 min, and the optimum temperature 30 ℃ , for carboxylesterase in poplar. Furthermore, the effect on carboxylesterase from poplar by omethoate, imidacloprid and fipronil was determined. The results showed that carboxylesterase activity of poplar was inhibited by 400,1 600,3 200 and 6 400 mg/mL omethoate, and the inhibition effect of 6 400 mg/mL omethoate was strongest. The carboxylesterase activity of poplar didn't change, after being treated by 50,100,200,400 and 800 mg/mL imidacloprid for 48 h, and the activity decreased by 400 and 800 mg/mL imidacloprid for 96 h. The carboxylesterase activity of poplar decreased after being treated by 400 mg/mL fipronil for 48 h, and by 800 mg/mL fipronil for 96 h.关键词
美洲黑杨/氧化乐果/吡虫啉/氟虫腈/羧酸酯酶活性Key words
poplar/ omethoate/ imidacloprid/ fipronil/ carboxylesterase activity分类
农业科技引用本文复制引用
汤方,朱莹嘉..3种杀虫剂对杨树羧酸酯酶的抑制效果[J].南京林业大学学报（自然科学版）,2012,36(2):81-85,5.基金项目
国家自然科学基金项目(30972376) （30972376）
霍英东青年教师奖(123086) （123086）
