南京林业大学学报（自然科学版）2012，Vol.36Issue(3)：17-22,6.
慈竹CCoAOMT基因的克隆及生物信息学分析
Cloning of CCoAOMT gene in Neosinocalamus affinis and its bioinformatics analysis
摘要
Abstract
Caffeoyl-coA O-methyltransferase (CCoAOMT) is a key enzyme in ligntn biosynthesis. A gene of CCoAOMT family with the cDNA length of 1 080 bp was cloned by RT-PCR and RACE method from young stems of Neosinocalamus affinis and bioinformatics analyzed. The full-length sequence of the CCoAOMT gene from N. affinis was successfully cloned in this studies. The results showed that the gene contained an open reading frame of 777 bp, which encoded a polypeptide of 258 amino acids, the molecular weight of the predicted protein was 28.861 ku, its theoretical PI was 5. 33, the gene's secondary structure consist of ot-helix, β-turn, extended strand and random coil; the proportions were 42.25 % ,8.91 % , 32.95 % and 15.89 % , respectively. The BLASTp results indicated that N. affinis had the highest identity with Bambusa oldhamii. There is a base deletion at the tenth site, and has five variable sites compared with CCoAOMT of B. oldhamii. One relative conserved disordered domain was found in the CCoAOMT gene from N. affinis, B. oldhamii, Phyllostachys edulis and Zea mays. The expression of NaCCoAOMTl showed that the gene highly expressed in young leaves, upper and middle parts of shoots and stem. This is the first time that NaCCoAOMTl was cloned in N. affinis. The sequence of NaCCoAOMTl has been submitted to GenBank with the accession number JF742462.关键词
慈竹/CCoAOMT/基因克隆/生物信息学分析Key words
Neosinocalamus affinis/ caffeoyl-coA O-methyltransferase/ gene clone/ bioinformatics analysis分类
农业科技引用本文复制引用
吴晓宇,胡尚连,曹颖,卢学琴,任鹏,周美娟,李晓瑞..慈竹CCoAOMT基因的克隆及生物信息学分析[J].南京林业大学学报（自然科学版）,2012,36(3):17-22,6.基金项目
四川省"十二五"重点攻关项目(2011YZGG-10) （2011YZGG-10）
四川省应用基础研究基金项目(05JY029-101) （05JY029-101）
四川主要丛生竹定向培育技术项目(10zx1102) （10zx1102）
