南京农业大学学报2012，Vol.35Issue(3)：13-18,6.
葡萄未成熟胚诱导体细胞胚发生和植株再生与遗传鉴定
Somatic embryogenesis from immature zygotic embryos and genetic fidelity identificating of regenerated plants of grapevine
摘要
Abstract
Somatic embryogenesis and plant regeneration were successfully established on Nitsch and Nitsch ( NN69) medium from immature zygotic embryos(IZEs) of interploid crossing 2004-6-12 × 'Aki Queen'. The optimum hormone combinations was NN69 + 1.80 mg?L-1 NAA+0.4 mg?L-1 casein hydroly sale+2 g?L-1 activated carbon + 30 g?L-1 sucrose+6 g?L-1 agar(SE medium)for primary somatic embryogenesis directly from IZEs, with the frequency varying from 11. 11% to 16.67% among IZEs of different sampling period after pollination. And importantly, somatic embryogenesis and normal germinating occurred synchronically. While the optimum medium for secondary somatic embryogenesis were 3/4MS + 0. 35 mg ? L-1 IBA + 30 g ? L-1 sucrose + 6 g ? L-1 agar (multiplication medium)and MS+1.5 g?L-1 6-BA+0.2 g?L-1 IBA+30 g?I-1 sucrose+6 g?L-1 agar(somatic embryo germination medium). After subcultured on germination medium, somatic embryos converted into normal plantlets. The analysis of DNA content and genetic fidelity determined by flow cytometry and SSR of the regenerated plantlets clearly indicated that the four plantlets were triploid and the normal germinated plant and the somatic embryogenesis-derived plants came from the same immature zygotic embryos. We concluded that somaclonal variation was almost absent in our grapevine plant regeneration system, which was useful in research of transformation and embryo development and so on.关键词
葡萄/未成熟合子胚/体细胞胚发生/流式细胞仪/SSRKey words
grapevine/immature zygotic embryos/somatic embryogenesis/flow cytometry/SSR分类
农业科技引用本文复制引用
孙兴民,余智莹,张萌,董瑞奇,陶建敏..葡萄未成熟胚诱导体细胞胚发生和植株再生与遗传鉴定[J].南京农业大学学报,2012,35(3):13-18,6.基金项目
国家948项目(2011-G28) （2011-G28）
江苏省农业科技自主创新资金项目(cx(10)110) （cx(10）
江苏省农业资源开发局项目(2012-kj59) （2012-kj59）
