南京农业大学学报2012，Vol.35Issue(3)：42-46,5.
甘蓝型油菜‘南盐油1号’不同外植体再生体系的比较研究
The comparative study on different explant regeneration systems in canola‘ Nanyanyou 1' (Brassica napus L.)
摘要
Abstract
The plant tissue culture was tried in the experiment,in which hypocotyl and the cotyledon with petiole of 'Nanyanyou 1' ( Brassica napus L. ) were used as materials. The effects of cross combination of the different pre-culture and differentiation culture on the canola plant regeneration were studied. The results showed that different concentrations of 2,4-D,6-BA and AgNO3 had varing degree of influences on explant differentiation,however,differentiated buds were all able to take root and grow into plant. The differentiation rate of hypocotyl achieved the peak value(95.23% )in the pre-culture( MS+0.5 mg ? L-12,4-D+1.0 mg ? L-1 6-BA)and differentiation ( MS+3.0 mg ? L-1 6-BA+0.1 mg ? L-1 NAA+5.0 mg ? L-1 AgNO3);the cotyledon with petiole achieved the peak value (89.52%)in pre-culture(MS+0. 5 mg ? L-1 2,4-D+1.0 mg ? L-1 6-BA)and differentiation( MS+4.5 mg ? L-1 6-BA+0.1 mg ? L-1 NAA+5.0 mg ? L-1 AgNO3).To sum up,compared with the cotyledon with petiole,hypocotyl might be more suitable as the explant in tissue culture research of Nanyanyou 1'.关键词
甘蓝型油菜/下胚轴/具柄子叶/再生体系Key words
Brassica napus L./ hypocotyl/cotyledon with petiole/regeneralion system分类
农业科技引用本文复制引用
刘国红,梁明祥,周诗雨,郑青松,刘兆普..甘蓝型油菜‘南盐油1号’不同外植体再生体系的比较研究[J].南京农业大学学报,2012,35(3):42-46,5.基金项目
国家科技支撑计划项目(2011BAD13B09) （2011BAD13B09）
作物遗传与种质创新国家重点实验室开放课题(ZW2010004) （ZW2010004）
校青年科技创新基金项目(Y201058) （Y201058）
