南京农业大学学报2012，Vol.35Issue(3)：52-56,5.
禾本科植物内生真菌研究13:禾本科植物内生真菌的分离鉴定及基因组DNA的快速提取
Grass endophytic fungi researches 13 :Endophytic fungi identification and rapid extraction of genomic DNA
摘要
Abstract
Endophytic fungi were surveyed in 12 different regions of 7 provinces in China,and 2 Epichlo(e) species and 4 Neotyphodium species were identified. In this study,we used improved method for genomic DNA extraction from slow-growing fungal endophytes and validated it by PCR. The IQS( improved quick and safe)method was achieved by using plate-grown mycelia as the starting material. This new method was in virtue of simple equipment, easy operation, rapid and safe extraction, and low cost. The whole extraction process could be accomplished in 40 minutes. It took 2 weeks which was 1/4 of SDS procedures. Then DNA was used as template to detect the NRPS genes of the endophytes. The resulted sequences had a high sequence similarity with the NRPS gene. The quality of DNA samples from this method could be used for subsequent PCR, cloning, and other high-throughput testing requirements, particularly suitable for a large number of fungal strains.关键词
禾本科植物内生真菌/基因组DNA的提取/NRPS基因/PCR扩增/序列比对Key words
grass endophyles/extraction of genomic DNA/NRPS gene/PCR amplification/alignment分类
生物科学引用本文复制引用
王永,纪燕玲,王晗,陈永敢,王志伟..禾本科植物内生真菌研究13:禾本科植物内生真菌的分离鉴定及基因组DNA的快速提取[J].南京农业大学学报,2012,35(3):52-56,5.基金项目
国家自然科学基金项目(30670008,30800156,30970081) （30670008,30800156,30970081）
