南京农业大学学报2012，Vol.35Issue(3)：64-70,7.
转群体感应淬灭基因attM烟草抗青枯病的研究
Increasing resistance to Ralstonia solanacearum in transgenic tobacco by expressing a quorum-qunching gene attM
摘要
Abstract
The attM fragment was cloned from Agrobacterium tumefaciens strain EHA105.The calli, produced from tobacco leaf discs co-cullured with attM gene mediated by A. tumefaciens,were exposed to 100 mg ? L-1 kanamycin for selection,and the survival of the calli were numhered and microprogated. The presence of attM gene in the plant material was demonstrated by PCR, PCR-Southern,qPCR and fluorometric GUS respectively. The relative expression quantification of attM in different transgenic tobacco plant lines were 31.0,the minimum and 125.8,the maximum, that the transgenic tobacco plants had successfully obtained resistance to bacterial wilt caused by Ralstonia solanacearum was comfirmed in vitro and in vivo. When R. solanacearum inoculated at the concentration of 1×107-1×108 CFU ? mL-1 on the leaves in vitro,the ratio of pathogenic area to inocated piont was from 15 to 25 in the wild type and pB1121-vector transferred plants,but that in transgenic plants was less than 3. Fourteen days after root inoculation in vivo at 3×106 CFU ? mL-1 on the potted plants,the wild type and pBI121-vector transferred plants showed wilt all with the disease index 100,however,the transgenic plants showed only some basal leaves wilt with the disease index 31.25.关键词
转attM基因烟草/青枯病/抗病性Key words
attM transgenic tobacco/bacterial wilt caused by Ralstonia solanacearum/resistance分类
农业科技引用本文复制引用
牛毅,李婷,谷春艳,杨雪,钱国良,胡白石,刘凤权,范加勤..转群体感应淬灭基因attM烟草抗青枯病的研究[J].南京农业大学学报,2012,35(3):64-70,7.基金项目
江苏省自然科学基金项目(BK2010445) （BK2010445）
江苏高校优势学科建设工程资助项目 （）
