南京农业大学学报2012，Vol.35Issue(3)：89-93,5.
Kisspeptin-10对鹌鹑开产及胆固醇代谢的影响
Effects of kisspeptin-10 on the onset of egg laying and cholesterol metabolism in quails
摘要
Abstract
In order to investigate the effect of kisspeptin-10( Kp-10)on the onset of egg laying and lipids metabolism in birds,75 female quails(22 days of age) were subjected to 0( control ,C) ,0.1 nmol( low dosage, L group)and 1.0 nmol( high dosage,H group) Kp-10 administration by inlraperitoneal injection daily. The experimental time has lasted for 21 days. Kp-10 was disolved in saline and the total volume for injection was 300 μL. The information of egg laying was recorded daily. At 60 days of age, quails were sacrificed and samples including serum and liver were collected for analysis. The concentration of estrogen (E2) in serum and cholesterol metabolism related genes expression in liver, were measured respectively. The results showed that Kp-10 treatment under low as well as high dosage significantly increased the egg laying rate and advanced the onset of egg laying of quails( P<0.05 ). Serum E2 concentration was increased by Kp-10 treatment on dose dependent manner and reached the statistically significance in H group of quails(P<0. 05 ). Compared with the control group, the concentration of total cholesterol ( Tch) and high density lipoprotein cholesterol ( HDL-C ) in serum of quails in H group significantly decreased (P<0.05 ) , however, the level of Tch in liver showed a significant increase in H group ( P < 0. 05 ). Real-time RT-PCR results showed that cholesterol-7alpha-hydroxylase ( CYP7A1) gene transcription in liver was significantly up-regulated by high but not low dosage of Kp-10 treatment(P<0.01). The gene expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase ( HMGCR) was increased by Kp-10 treatment but did not reach the statistical significance(/3>0. 05). In addition,compared to the control group, there was no significant change of sterol regulatory element-binding protein 2 ( SREBP-2) mRNA transcription or protein expression in liver of quails (P>0. 05). These results indicated that exogenous kisspeptin injection can advance egg laying onset rate of quails and increase lipogenesis in liver.关键词
kispeptin-10/开产率/胆固醇/肝脏/鹌鹑Key words
kisspeptin-10/laying rate/eholesterol/liver/quail分类
农业科技引用本文复制引用
吴晶,詹年,谢怀东,倪迎冬,赵茹茜..Kisspeptin-10对鹌鹑开产及胆固醇代谢的影响[J].南京农业大学学报,2012,35(3):89-93,5.基金项目
国家自然科学基金项目(30800809) （30800809）
