南京农业大学学报2012，Vol.35Issue(3)：94-100,7.
A/J小鼠感染猪链球菌2型后脾脏消减cDNA文库的构建及相关差异基因表达分析
Construction of a suppression subtraction hybridization cDNA library to screen differentially expressed genes and functional analysis from the spleen of A/J mouse strains with infected Streptococcus suis type 2
摘要
Abstract
This study aimed to screen the differentially expressed genes related to the infection of Streptococcus suis type 2 ( SS2). The cDNA library were constructed from the 8-week-old age spleen of A/J mouse strains infected to SS2 by suppression subtraction hybridization ( SSH). A total of 169 positive clones were sequenced. After dislodging, clustering and splicing the redundant cDNA sequences, a total of 110 differentially expressed sequence tags(EST) were obtained in the whole subtractive cDNA library. We used the software BLAST in NCBl of GenBank to do the homology comparisons of nucleic acid and protein. The results showed that 36 differentially expressed genes had highly homologous with EST database. Those gene fragments involved the cell components, immunity, cell signal transduclion,apoptosis,transeriptional factor genes and so on.The expression of ATP/GTP binding protein 1(Agtpbpl), Asp-Glu-Ala-Asp box polypeptide 5, nucleoprolein-p68, ATP-dependent RNA helicase, eukaryotic translation initiation factor 2 subunit 2 ( Eif2s2 ), ATPase/Na+/K+ transporting β3 polypeptide ( Atpl β3 ) , lysozyme 1 ( Lyzl ) and lysozyme 2 ( Lyz2 ) were significantly different after the infection of SS2 in the spleen of A/J mouse. The results indicated that those genes played an important part in the infection of SS2.关键词
A/J小鼠:抑制性消减杂交/差异表达基因/消减cDNA文库Key words
A/J mouse strain/ supperession subtraction hybridization (SSH) / differentially expressed genes /subtraetive cDNA library分类
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谢正露,沈学怀,殷复建,刘琳,马海田,范红结..A/J小鼠感染猪链球菌2型后脾脏消减cDNA文库的构建及相关差异基因表达分析[J].南京农业大学学报,2012,35(3):94-100,7.基金项目
转基因生物新品种培育重大专项(2009ZX08009-1548) （2009ZX08009-1548）
