南京农业大学学报2012，Vol.35Issue(3)：107-113,7.
转绵羊IRF-1基因牛胎儿成纤维细胞的BVDV抗性研究
Study on the anti-BVDV activity of ovis aries IRF-1 transfected bovine fetal fibroblast cells
摘要
Abstract
To evaluate the antivirus efficiency of IRF-1, transfected bovine fetal fibroblast(BFF) that transiently expressed IRF-1 were prepared,and then the above cells were treated with bovine viral diarrhea virus(BVDV) which belonged to positive-strand RNA virus. Cells were examined on their TCID50 cell morphology,growth curve,apoptosis and gene expression level. The results showed that compared to non-transfected cells,the TCID50 on the transgenic cells was significantly higher,the cytopathic effect(CPE) of the transgenic cells was remarkably alleviated at 48 and 72 h, and the transfected cells' apoptosis rate was also significantly lower by the flow cytometric analysis,whereas the expression levels of IRF-1 was remarkable higher in the transgenic cells. The results indicated that IRF-1 played an important role in BVDV control.关键词
IRF-1/转基因/牛胎儿成纤维细胞(BFF)/BVDV病毒:抗病毒Key words
IRF-1/transgene/bovine fetal fibroblast(BFF)/ bovine viral diarrhea virus(BVDV) /antivirus分类
农业科技引用本文复制引用
齐巍巍,唐泰山,王子玉,闫益波,钟部帅,吴勇聪,张常印,茆达干,王锋..转绵羊IRF-1基因牛胎儿成纤维细胞的BVDV抗性研究[J].南京农业大学学报,2012,35(3):107-113,7.基金项目
转基因生物新品种培育重大专项(2008ZX08008-005) （2008ZX08008-005）
