南京医科大学学报（自然科学版）2012，Vol.32Issue(4)：443-448,6.
Zn缺乏对人精子VDAC mRNA的影响
Effects of intracellular zinc depletion on the abundance of VDAC mRNA in cultured human spermatozoa
摘要
Abstract
Objective:To investigate whether intracellular zinc depletion can actually change spermatozoa motiJity and the abundance of voltage-dependent anion channel (VDAC) mRNA in cultured human spermatozoa. Methods; Spermatozoa were separated from ejaculates of three donors using a discontinuous Percoll gradient centrifugation. Each spermatozoa specimen were divided into 3 groups,one was exposed to a cell membrane-permeable zinc chelator N,N,N',N'-tetrakis (2-pyridylmethyl) Ethylenediamine (TPEN) (2 μmol/L) for 24 hours,one was to TPEN plus zinc sulfate (5 μmol/L) for 24 hours,and one was as control. CASA and quantitative real-time PCR (qPCR) were performed to detect sperm motility characteristics,mRNA abundance and difference of the three VDAC subtypes between three different treatment groups respectively. Results;The results of CASA demonstrated that exposure of spermatozoa to TPEN for three hours significantly decreased sperm motility compared with the control group (P = 0.049). qPCR demonstrated that VDAC3 mRNA level of TPEN group were significantly lower than that of control group,while no significant difference was found between TPEN plus zinc sulfate treatment group and control group. Co-addition of zinc partly reversed TPEN-induced alterations of sperm motility and VDAC3 mRNA abundance. Conclusion;The results of the present study implicate that zinc depletion may induce VDAC3 mRNA abundance decreasing and affect the normal function of spermatozoa.关键词
Zn/精子/VDAC/男性不育/Zn缺乏Key words
Zn/ sperm/ VDAC/male infertility/zinc depletion分类
医药卫生引用本文复制引用
赵丹,吴炜,章轶,王增军,王心如..Zn缺乏对人精子VDAC mRNA的影响[J].南京医科大学学报（自然科学版）,2012,32(4):443-448,6.基金项目
国家自然科学基金面上项目(30872575,30973199) （30872575,30973199）
