南京医科大学学报（自然科学版）2012，Vol.32Issue(4)：509-513,5.
糖尿病患者外周血FOXP3+调节性T细胞的检测及其临床意义
Percentages of FOXP3+ regulatory T cells in patients with diabetes
摘要
Abstract
Objective: To analyze the percentage of F0XP3+ regulatory T cells in peripheral blood from patients' with diabetes of different types. Methods; Three groups were included;type 1 diabetes (n = 43 cases) ,type 2 diabetes (n = 16 cases) and healthy control (n = 19 cases). Zinc transporter 8 antibody (ZnT8A),glutamic acid decarboxylase antibody (GADA) and islet cell antibody (ICA) were detected by radioligand binding assay. The proportions of regulatory T cells,CD4+CD25+FOXP3+T cells and CD4+CD25+T cells,in peripheral blood were determined by flow cytometry. Results: ① In the peripheral blood,the percentages of CD4+CD25+T cells in CD4+T lymphocytes from patients with type 1 diabetes or type 2 diabetes were lower than those of healthy control; ② In the peripheral blood,patients with type 1 diabetes showed lower ratio of CD4+ CD25+ F0XP3+ T cells compared with healthy control and patients with type 2 diabetes;③ There was no significant correlation between the duration of diabetes and the percentage of peripheral CD4+CD25+FOXP3+ regulatory T cells or CD4+CD25+T cells. Conclusion: In the peripheral blood,the FOXP3+ regulatory T cells population was deficiency in patients with type 1 diabetes. Type 2 diabetes had small influence in the percentage of F0XP3* regulatory T cells. There was no significant relationship between the percentage of FOXP3+ regulatory T cells and duration of type 1 diabetes.关键词
调节性T淋巴细胞/糖尿病/流式细胞术Key words
regulatory T cell/diabetes/flow cytometry分类
医药卫生引用本文复制引用
张小娇,孔璐璐,顾榕,陈恒,许馨予,徐宽枫,王知笑,杨涛,张梅..糖尿病患者外周血FOXP3+调节性T细胞的检测及其临床意义[J].南京医科大学学报（自然科学版）,2012,32(4):509-513,5.基金项目
国家自然科学基金(81070622) （81070622）
江苏省自然科学基金(BK2008444) （BK2008444）
南京市科技发展项目(009010684) （009010684）
江苏六大人才高峰项目(2010-022) （2010-022）
