南京医科大学学报（自然科学版）2012，Vol.32Issue(4)：577-581,5.
运用Identifiler试剂盒对硅珠法提取石蜡包埋组织DNA质量的评估
Using Identifiler Kit evaluates silica extracting DNA from paraffin-embedded tissue
摘要
Abstract
Objective;To extract DNA from paraffin-embedded tissue by silica-extraction. Methods;The DNA samples were obtained via silica-extraction method from 49 cases of paraffin-embedded tumor tissues from Department of Pathology in the First Affiliated Hospital of NJMU, The 16 STR gene loci of PCR-DNA fragments were identified with Identifier Kit The purity of DNA extracted was analyzed by ultraviolet spectrophotometer. Results;The mean D (260 nm)/D (280 nm) of DNA extracted from gastric carcinoma was 1.84 ± 0.02,from pulmonary carcinoma is 1.85 ± 0.02,from thyroid carcinoma was 1.82 ± 0.02. There was no significant difference in D(260 nm)/D(280 nm) among three carcinoma type groups(P > 0.05). The 46/49 cases(93%) showed the full peaks of all the 16 STR gene loci of PCR-DNA fragment Conclusion;Silica-extraction may be used to various kinds of paraffin-embedded tissues and extract DNA with high purity and high integrity.关键词
石蜡包埋组织/硅珠法/Identifiler试剂盒/DNAKey words
paraffin-embedded tissue/ silica-extraction/ Identifier Kit/ DNA分类
医药卫生引用本文复制引用
林旭,王建文,尹雅莎,谢素梅,冀强..运用Identifiler试剂盒对硅珠法提取石蜡包埋组织DNA质量的评估[J].南京医科大学学报（自然科学版）,2012,32(4):577-581,5.基金项目
江苏省教育厅科学基金资助(05KJD310140) （05KJD310140）
